The surface force apparatus (SFA) was utilized to obtain force-distance profiles between silica-supported membranes formed by Langmuir-Blodgett deposition of 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). In the absence of a membrane, a long-range electrostatic repulsion and short-range steric repulsion are measured as a result of the deprotonation of silica in water and the roughness of the silica film. The electrostatic repulsion is partially screened by the lipid membrane, and a van der Waals adhesion comparable to that measured with well-packed DPPC membranes on mica is measured. This finding suggest that electrostatic interactions due to the underlying negatively charged silica are likely present in other systems of glass-supported membranes. In contrast, the charge of an underlying mica substrate is almost completely screened when a lipid membrane is deposited on the mica. The difference in the two systems is attributed to the stronger physisorption of zwitterionic lipids to molecularly smooth mica compared to physisorption to rougher silica.