Rapid and simple colorimetric assay for detecting the enzymatic degradation of biodegradable plastic films

Shinozaki Y; Watanabe T; Nakajima-Kambe T; Kitamoto HK

Journal of Bioscience and Bioengineering, Vol.115, No.1, 111-114, 2013

DOI10.1016/j.jbiosc.2012.08.010 Export Citation

Rapid and simple colorimetric assay for detecting the enzymatic degradation of biodegradable plastic films

Shinozaki Y, Watanabe T, Nakajima-Kambe T, Kitamoto HK

We developed a rapid and simple method for evaluating the degradation of solid biodegradable plastics (BPS). Dye-containing BP films were used as substrates and the release of dye caused by the degradation of BPs was confirmed by a color change in the enzyme solution after a reaction time of 24 h. (C) 2012, The Society for Biotechnology, Japan. All rights reserved.

Keywords:Biodegradation;Poly(butylene succinate-co-adipate);Poly(lactic acid);Polyurethane;Pseudozyma antarctica