Applied Biochemistry and Biotechnology, Vol.169, No.1, 301-311, 2013
Production of Metabolites with Antioxidant and Emulsifying Properties by Antarctic Strain Sporobolomyces salmonicolor AL(1)
The Sporobolomyces salmonicolor AL(1) Antarctic strain was cultivated and two bioproducts were obtained: exopolysaccharide and biomass. The biologically active substances ergosterol, torularhodin, torulene, beta-carotene and CoQ(10) were extracted from the biomass and were quantified as follows: ergosterol 5.2 +/- 0.2 mg/g, torularhodin 458.3 +/- 24.5 mu g/g, torulene 273.7 +/- 14.5 mu g/g, beta-carotene 129.2 +/- 7.3 mu g/g and coenzyme Q(10) (CoQ(10)) 236.1 +/- 12.1 mu g/g. Their antioxidant activity was estimated according to the cathode voltammetry method. The most pronounced antioxidant activity (according to trolox) was exhibited by beta-carotene 3.78, followed by CoQ(10) 3.60, both of them being the main contributors to the total extract activity of 3.19. The biologically active metabolites in combination with exoglucomannan as emulsifier were used for the creation of model emulsion systems characterised by great stability. The absorption of UVA rays by the model emulsions was studied.
Keywords:S. salmonicolor AL(1);Carotenoids;Coenzyme Q(10);Ergosterol;Antioxidant activity;Exopolysaccharide