Synergistic effect of pretreatment and hydrolysis enzymes on the production of fermentable sugars from date palm lignocellulosic waste

Sulaiman Al-Zuhair; Khalda Ahmed; Ashir Abdulrazak; Muftah H. El-Naas

Journal of Industrial and Engineering Chemistry, Vol.19, No.2, 413-415, March, 2013

DOI10.1016/j.jiec.2012.09.022 Export Citation

Synergistic effect of pretreatment and hydrolysis enzymes on the production of fermentable sugars from date palm lignocellulosic waste

Sulaiman Al-Zuhair^†, Khalda Ahmed, Ashir Abdulrazak, and Muftah H. El-Naas

Chemical and Petroleum Engineering Department, UAE University, 17555 Al-Ain, United Arab Emirates

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The sequential addition of the enzymes, laccase for lignin degrading, followed by xylanase for hemicelluloses hydrolysis, then cellulase for cellulose hydrolysis, was compared to the synergistic action of using the three enzymes together. It was shown that the reducing sugars yield increased from 5.6% using cellulase only to 45.6% by pretreatment with laccase and xylanase, prior to the enzymatic hydrolysis. A higher conversion of 60% was achieved by using the three enzymes together for the same incubation period. The proposed synergistic enzymes approach is a simpler and less energy intensive alternative compared to the conventional lignocelluloses pretreatment techniques.

Keywords:Enzymatic pretreatment;Lignocelluloses;Synergic effect;Reducing sugars

[References]

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[1] Klemm D, Heublein B, Fink H, Bohn A, Angewandte Chemie., 44, 3358 (2005)

[2] Hendriks ATWM, Zeeman G, Bioresour. Technol., 100(1), 10 (2009)

[3] Sun Y, Cheng JY, Bioresour. Technol., 83(1), 1 (2002)

[4] Bura R, Mansfield SD, Saddler JN, Bothast RJ, Applied Biochemistry and Biotechnology., 98, 59 (2002)

[5] McMillan JD, in: Himmel ME, Baker JO, Overend RP (Eds.), Enzymatic Conversion of Biomass for Fuels Production, American Chemical Society, Washington, DC, 292 (1994)

[6] Arora DS, Chander M, Gill PK, International Biodeterioration and Biodegradation., 50, 115 (2002)

[7] Moilanen U, Kellock M, Galkin S, Viikari L, Enzyme Microb. Technol., 49(6-7), 492 (2011)

[8] Ibrahim V, Mendoza L, Mamo G, Hatti-Kaul R, Process Biochemistry., 46, 379 (2011)

[9] Srebotnik E, Hammel KE, Biotechnology., 81, 179 (2000)

[10] Menon V, Prakash G, Prabhune A, Rao M, Bioresour. Technol., 101(14), 5366 (2010)

[11] Shen F, Kumar L, Hu JG, Saddler JN, Bioresour. Technol., 102(19), 8945 (2011)

[12] Bajpai P, Biotechnol. Prog., 15(2), 147 (1999)

[13] Barbosa AM, Dekker RFH, Kurtbo¨ ke I, Hardy G, Letters in Applied Microbiology., 23, 93 (1996)

[14] Ghose TK, Bisaria VS, Pure and Applied Chemistry., 59, 1739 (1987)

[15] Hankin L, Anagnostakis SL, Mycologia., 67, 67 (1975)

[16] Miller GL, Analytical Chemistry., 31, 426 (1959)