Sepantronium Bromide (YM155) induces disruption of the ILF3/p54(nrb) complex, which is required for survivin expression

Yamauchi T; Nakamura N; Hiramoto M; Yuri M; Yokota H; Naitou M; Takeuchi M; Yamanaka K; Kita A; Nakahara T; Kinoyama I; Matsuhisa A; Kaneko N; Koutoku H; Sasamata M; Kobori M; Katou M; Tawara S; Kawabata S; Furuichi K

Biochemical and Biophysical Research Communications, Vol.425, No.4, 711-716, 2012

DOI10.1016/j.bbrc.2012.07.103 Export Citation

Sepantronium Bromide (YM155) induces disruption of the ILF3/p54(nrb) complex, which is required for survivin expression

Yamauchi T, Nakamura N, Hiramoto M, Yuri M, Yokota H, Naitou M, Takeuchi M, Yamanaka K, Kita A, Nakahara T, Kinoyama I, Matsuhisa A, Kaneko N, Koutoku H, Sasamata M, Kobori M, Katou M, Tawara S, Kawabata S, Furuichi K

YM155, a small-molecule survivin suppressant, specifically binds to the transcription factor ILF3, which regulates the expression of survivin [1]. In this experiment we have demonstrated that p54(nrb) binds to the survivin promoter and regulates survivin expression. p54(nrb) forms a complex with ILF3, which directly binds to YM155. YM155 induces disruption of the ILF3/p54(nrb) complex, which results in a different subcellular localization between ILF3 and p54(nrb). Thus, identification of molecular targets of YM155 in suppression of the survivin pathway, might lead to development of its use as a novel potential target in cancers. (C) 2012 Elsevier Inc. All rights reserved.

Keywords:YM155;survivin expression;p54(nrb);ILF3