Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes

Nevena Z. Prlainovic; Dejan I. Bezbradica; Zorica D. Knezevic-Jugovic; Sanja I. Stevanovic; Milka L. Avramov Ivic; Petar S. Uskokovic; Dusan Z. Mijin

Journal of Industrial and Engineering Chemistry, Vol.19, No.1, 279-285, January, 2013

DOI10.1016/j.jiec.2012.08.012 Export Citation

Adsorption of lipase from Candida rugosa on multi walled carbon nanotubes

Nevena Z. Prlainovic^†, Dejan I. Bezbradica, Zorica D. Knezevic-Jugovic, Sanja I. Stevanovic¹, Milka L. Avramov Ivic¹, Petar S. Uskokovic, and Dusan Z. Mijin

Faculty of Technology and Metallurgy, University of Belgrade, Karnegijeva 4, Belgrade, Serbia
¹ICTM, Institute of Electrochemistry, University of Belgrade, Njegosˇeva 12, Belgrade, Serbia

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In this work lipase from Candida rugosa was adsorbed on unmodified surface of multi walled carbon nanotubes (raw-MWCNT). The effects of immobilization time, initial enzyme concentration and buffer ionic strength on enzyme loading and activity of immobilized preparations were tested. High loadings are attained. The immobilized enzyme obtained at lowest initial enzyme concentration and high ionic strength retained 85% of initial enzyme activity. It is assumed that immobilization on hydrophobic surface led to conformational changes that resulted in the adsorption of lipase in active conformation. Immobilized preparations were characterized, with FT-IR spectroscopy, AFM, and cyclic voltammetry.

Keywords:Lipase;Carbon nanotubes;Adsorption;FT-IR spectroscopy;AFM;Cyclic voltammetry

[References]

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[Referenced By]

[1] Sheldon RA, Advanced Synthesis and Catalysis., 349, 1289 (2007)

[2] Spahn C, Minteer SD, Recent Patents on Engineering., 2, 195 (2008)

[3] Liang JF, Li YT, Yang VC, Journal of Pharmaceutical Sciences., 89, 979 (2000)

[4] Khan AA, Alzohairy MA, Research Journal of Biological Sciences., 5, 565 (2010)

[5] Tischer W, Wedekind F, Topics in Current Chemistry., 200, 95 (1999)

[6] Worsfold PJ, Pure and Applied Chemistry., 67, 597 (1995)

[7] Gao Y, Kyratzis I, Bioconjugate Chemistry., 19, 1945 (2008)

[8] Govardhan CP, Current Opinion in Biotechnology., 10, 331 (1999)

[9] Kim BC, Nair S, Kim J, Kwak JH, Grate JW, Kim SH, Gu MB, Nanotechnology., 16, S382 (2005)

[10] Asuri P, Karajanagi SS, Sellitto E, Kim DY, Kane RS, Dordick JS, Biotechnol. Bioeng., 95(5), 804 (2006)

[11] Palwai NR, Martyn DE, Neves LFF, Tan Y, Resasco DE, Harrison RG, Nanotechnology., 235601 (5 pp.), 18 (2007)

[12] Shah S, Solanki K, Gupta MN, Chemistry Central Journal., 1, 30 (2007)

[13] Jiang K, Schadler LS, Siegel RW, Zhang X, Zhangc H, Terronesd M, Journal of Materials Chemistry., 14, 37 (2004)

[14] Huang WJ, Taylor S, Fu KF, Lin Y, Zhang DH, Hanks TW, Rao AM, Sun YP, Nano Letters., 2, 311 (2002)

[15] de Oliveira PC, Alves GM, de Castro HF, Biochemical Engineering Journal., 5, 63 (2000)

[16] Schmitt J, Brocca S, Schmid RD, Pleiss J, Protein Engineering., 15, 595 (2002)

[17] Jeganathan J, Bassi A, Nakha G, J. Hazard. Mater., 137(1), 121 (2006)

[18] Gotor-Ferna´ ndez V, Brieva R, Gotor V, Journal of Molecular Catalysis B: Enzymatic., 40, 111 (2006)

[19] Murty VR, Bhat J, Muniswaran PKA, Biotechnology and Bioprocess Engineering., 7, 57 (2002)

[20] Fernandez-Lafuente R, Armisen P, Sabuquillo V, Fernandez-Lorente G, Guisan JM, Chemistry and Physics of Lipids., 93, 185 (1998)

[21] Guisan JM, Sabuquillo V, Fernandez-Lafuente R, Fernandez-Lorente G, Mateo C, Halling PJ, Kennedy D, Miyata E, Re D, Journal of Molecular Catalysis B:Enzymatic., 11, 817 (2001)

[22] Foresti ML, Alimenti GA, Ferreira ML, Enzyme Microb. Technol., 36(2-3), 338 (2005)

[23] Persson M, Mladenoska I, Wehtje E, Adlercreutz P, Enzyme Microb. Technol., 31(6), 833 (2002)

[24] Shi Q, Yang D, Su Y, Li J, Jiang Z, Jiang Y, Yuan W, Journal of Nanoparticle Research., 9, 1205 (2007)

[25] Pavlidis IV, Tsofusis T, Enotiadis A, Gournis D, Stamatis H, Advanced Engineering Materials., 12, B179 (2010)

[26] Lee HK, Lee JK, Kim MJ, Lee CJ, Bulletin of the Korean Chemical Society., 31, 650 (2010)

[27] Ji PJ, Tan HS, Xu X, Feng W, AIChE J., 56(11), 3005 (2010)

[28] Vukovic GD, Marinkovic AD, Colic M, Ristic MD, Aleksic R, Peric-Grujic AA, Uskokovic PS, Chem. Eng. J., 157(1), 238 (2010)

[29] Bradford M, Analytical Biochemistry., 72, 248 (1976)

[30] Ivic MLA, Petrovic SD, Mijin DZ, Zivkovic PM, Kosovic IM, Drjevic KM, Jovanovic MB, Electrochim. Acta, 51(12), 2407 (2006)

[31] Drljevic-Djuric KM, Jovic VD, Lacnjevac UC, Ivic MLA, Petrovic SD, Mijin DZ, Djordjevic SB, Electrochim. Acta, 56(1), 47 (2010)

[32] Wang J, Musameh M, Lin YH, J. Am. Chem. Soc., 125(9), 2408 (2003)

[33] Obradovic MD, Vukovic GD, Stevanovic SI, Panic VV, Uskokovic PS, Kowal A, Gojkovic SL, Journal of Electroanalytical Chemistry., 634, 22 (2009)

[34] Bolivar JM, Mateo C, Godoy C, Pessela BCC, Rodrigues DC, Giordano RLC, Fernandez-Lafuente R, Guisan JM, Process Biochemistry., 44, 757 (2009)

[35] Bastida A, Sabuquillo P, Armisen P, Fernandez-Lafuente R, Huguet J, Guisan JM, Biotechnol. Bioeng., 58(5), 486 (1998)

[36] Natalello A, Ami D, Brocca S, Lotti M, Doglia SM, Biochemical Journal., 385, 511 (2005)

[37] Grochulski P, Li Y, Schrag JD, Cygler M, Protein Science., 3, 82 (1994)

[38] Brocca S, Alberghina L, Lotti M, Persson M, Wehtje E, Adlercreutz P, Protein Science., 9, 985 (2000)

[39] Palomo JM, Munoz G, Fernandez-Lafuente G, Mateo C, Fernandez-Lafuente R, Guisan JM, Journal of Molecular Catalysis B: Enzymatic., 19-20, 279 (2002)

[40] Screen J, Stanca-Kaposta EC, Gamblin DP, Liu B, Macleod NA, Snoek LC, Davis BG, Simons JP, Angewandte Chemie International Edition., 46, 3644 (2007)