화학공학소재연구정보센터
홈 로그인 로그아웃 연락처 사이트맵
Korean Journal of Chemical Engineering, Vol.30, No.3, 671-674, March, 2013
DOI10.1007/s11814-012-0172-3  Export Citation
Dispersion stability of citrate- and PVP-AgNPs in biological media for cytotoxicity test
Jinkyu Roh, Ha Nee Umh, Jaehoon Sim, Sumin Park1, Jongheop Yi1, and Younghun Kim†
  • Department of Chemical Engineering, Kwangwoon University, Seoul 139-701, Korea
  • 1School of Chemical and Biological Engineering, Seoul National University, Seoul 151-742, Korea
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Silver nanoparticles (AgNPs) with antimicrobial property have been used for years in various applications, such as antibacterial coating and air/water purification to eliminate microorganisms. In recent years, a number of studies on the potential risk of nanomaterials in humans and the environment have appeared, and thus a large number of studies regarding the toxic effects of AgNPs on cells and micro-organisms have been reported. In in-vivo and invitro cytotoxicity tests, various biological media are used, and thus the dispersion stability of AgNPs in media is important to a successful toxicity test. Therefore, we investigated the dispersion stability of AgNPs in various biological media; PBS (phosphate buffered saline), FBS (fetal bovine serum), and de-ionized water (pH 2, 7, and 9) with and without photo-irradiation. Herein, citrate and PVP (polyvinylpyrrolidone) stabilized AgNPs were used as target materials. In short- and long-term stability tests, we found that PVP-AgNPs stabilized by steric hindrance have good dispersion stability in biological media, compared to citrate-AgNPs stabilized by electrostatic repulsion.
Keywords:Silver Nanoparticles;Cytotoxicity;Dispersion Stability;Citrate;PVP
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