A new flow path design for multidimensional protein identification technology using nano-liquid chromatography electrospray ionization mass spectrometry

Thangamani Rajesh; Hyung-Yeon Park; Eunjung Song; Changmin Sung; Sung-Hee Park; Jae-Hun Lee; Dongwon Yoo; Yun-Gon Kim; Jong-Min Jeon; Byung-Gee Kim; Yung-Hun Yang

Korean Journal of Chemical Engineering, Vol.30, No.2, 417-421, February, 2013

DOI10.1007/s11814-012-0134-9 Export Citation

A new flow path design for multidimensional protein identification technology using nano-liquid chromatography electrospray ionization mass spectrometry

Thangamani Rajesh¹, Hyung-Yeon Park², Eunjung Song³, Changmin Sung^{3, 4}, Sung-Hee Park^{3, 4}, Jae-Hun Lee^{3, 4}, Dongwon Yoo^{3, 5}, Yun-Gon Kim⁶, Jong-Min Jeon¹, Byung-Gee Kim^{3, 4}, and Yung-Hun Yang^{1, †}

¹Department of Microbial Engineering, College of Engineering, Konkuk University, 1, Hwayang-dong, Gwangjin-gu, Seoul 143-701, Korea
²Bio-MAX Institute, Seoul National University, Gwanak-gu, Seoul 151-742, Korea
³School of Chemical and Biological Engineering, Institute of Molecular Biology and Genetics, Seoul National University, Gwanak-gu, Seoul 151-742, Korea
⁴Interdisciplinary Program for Bioengineering, Seoul National University, Seoul 151-744, Korea
⁵, Korea
⁶Chemical Engineering, Soongsil University, 511, Sangdo-dong, Seoul 156-743, Korea

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Multidimensional protein identification technology (MudPIT) is one of the most versatile methods for separating and identifying highly complex peptides or proteins. However, there are still inherent problems resulting from salt in eluent systems and instrumentation with MudPIT. We designed a novel and simple flow path using twovalve system and successfully performed a fully automated peptide analysis using MudPIT coupled with nano-liquid chromatography electrospray ionization mass spectrometry (nLC-ESI-MS). It enables to generate a remarkably stable nanospray during the MudPIT analysis and realize the fully automated MudPIT system. This column arrangement could be easily installed to avoid laborious loading steps and unstable ionization from discontinuous flow. Consequently, the new flow path design for MudPIT system guarantees the detection of more peptides and higher protein coverage in proteome analysis.

Keywords:MudPIT;Nano LC-ESI-MS;SCX;C18;Peptide Identification

[References]

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[Referenced By]

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[1] Gorg A, Weiss W, Dunn MJ, Proteomics., 4, 3665 (2004)

[2] Aebersold R, Mann M, Nature., 422, 198 (2003)

[3] Hager JW, Anal. Bioanal. Chem., 378, 845 (2004)

[4] Tanaka K, Waki K, Ido Y, Akita S, Yoshida Y, Yoshida T, Rapid Commun. Mass Spectrom., 2, 151 (1988)

[5] Fenn JB, Mann M, Meng CK, Wong SF, Whitehouse CM, Science., 246, 64 (1989)

[6] Ducret A, Van Oostveen I, Eng JK, Yates JR, 3rd, Aebersold R, Protein Sci., 7, 706 (1998)

[7] Link AJ, Eng J, Schieltz DM, Carmack E, Mize GJ, Morris DR, Garvik BM, Yates JR, Nat. Biotechnol., 17, 676 (1999)

[8] Wolters DA, Washburn MP, Yates JR, 3rd, Anal. Chem., 73, 5683 (2001)

[9] Washburn MP, Wolters D, Yates JR, Nat. Biotechnol., 19, 242 (2001)

[10] Motoyama A, Venable JD, Ruse CI, Yates JR, 3rd, Anal.Chem., 78, 5109 (2006)

[11] Guzzetta AW, Chien AS, J. Proteome. Res., 4, 2412 (2005)

[12] Taylor P, Nielsen PA, Trelle MB, Horning OB, Andersen MB, Vorm O, Moran MF, Kislinger T, J. Proteome. Res., 8, 1610 (2009)

[13] Yang YH, Lee K, Jang KS, Kim YG, Park SH, Lee CS, Kim BG, Anal. Biochem., 387, 133 (2009)

[14] Kim YG, Shin DS, Yang YH, Gil GC, Park CG, Mimura Y, Cooper DK, Rudd PK, Dwek RA, Lee YS, Kim BG, Chem. Biol., 15, 215 (2008)

[15] Babcock MJ, Buttner MJ, Keler CH, Clarke BR, Morris RA, Lewis CG, Brawner ME, Gene., 196, 31 (1997)

[16] Severinov K, Mooney R, Darst SA, Landick R, J. Biol. Chem., 272, 24137 (1997)