The influence of substrate temperature and ambient gas pressure-composition on the characteristics of WO films synthesized by radio-frequency assisted pulsed laser deposition (RF-PLD) are studied with the aim to obtain nanostructured films with large surface area that appear promising for gas sensing applications. A tungsten target was ablated both in chemically reactive molecular oxygen at 5 Pa and in a mixed oxygen-helium atmosphere at 700 Pa. Corning glass was used as the substrate, at 473, 673 and 873 K. Other deposition parameters such as laser fluence (4.5 J/cm(2)), laser wavelength (355 nm), radio-frequency power (150 W), and target to substrate distance (4 cm) were kept fixed. The sensitivity on the deposition parameters of roughness, morphology, nanostructure and bond coordination of the deposited films were analysed by atomic force microscopy, scanning electron microscopy, transmission electron microscopy and micro-Raman spectroscopy. The role of the investigated process parameters to nanoparticle formation and to the development of an extended nanostructure is discussed. (C) 2009 Elsevier B.V. All rights reserved.