Fatigue tests have been performed for 18 silicone-rubber tubes operating as the active element of a crossflow pulsation generator. It was found that the silicone-rubber tube can be used as the main part of a cheap and reliable oscillator with good reproducibility of the downstream pressure. The operating life of the tubes was between 146 h and 378 h of continuous work for peak-to-peak downstream pressure amplitude 220 kPa to 310 kPa and frequency 5.2 Hz to 8.5 Hz.