An investigation was made into the dielectric spectroscopic characteristics of p-toluene sulfonate (PTS) doped polypyrrole (PPY) films in the presence and absence of immobilized glucose oxidase (GOX) in three different configurations : Al-PTS-PPY-Al, Al-PTS-PPY/GOX-Al, and Al-PTS-PPY/GOX/beta-D-glucose-Al, respectively. Measurement of dielectric loss and capacitance yielded valuable information about the dielectric properties of GOX immobilized in PTS doped PPY films. The effect of both the temperature and varying beta-D-glucose concentrations on the mobility of the charge carriers in these films was also systematically studied.