Poly(gamma-glutamic acid) (PGA) and poly(epsilon-lysine) (PL) solutions were used as components to prepare mixed hydrogels by gamma irradiation. A PGA and PL mixed solution was crosslinked to form a hydrogel with specific water content (weight of absorbed water/weight of dry gel) of 10-100 when the 5 wt % solution of mixed polymer was exposed to gamma radiation of 87 kGy dosage under N-2 atmosphere. The specific water content increased with increasing PGA content of the PGA/PL mixed gel. The influence of pH and salt concentration on equilibrium swelling was studied. A characteristic pH-sensitive swelling behavior was obtained using compositional changes of PGA and PL in the gel. PGA/PL 50/50 wt % mixed gel swelled in acid (pH < 4.0) and alkaline (pH > 6.0) conditions and was deswelled between pH 4.0 and 6.0 due to the ionic composition changes of the gel network. With an increase in the ratio of PGA to FL, the hydrogels showed increasing sensitivity to salt solutions (NaCl, Na2SO4, and CaCl2). In addition, degradation of PGA/PL gel by protease produced from Aspergillus oryzae was investigated at 40 degrees C and pH 7.0. PL gel was degraded completely within 2 days. An increase in the ratio of PGA in the PGA/PL mixed gel led to a decrease in the degree of degradation as expected. Some subtle degradation changes were found in the 50/50 and 80/20 wt % (PGA/PL) gels that were degraded by only 3.5 and 3.8% by protease, respectively.