New 2-aminomethoxy derivatives of 1-benzylthiohexane have been obtained via the Mannich condensation reaction of 1-benzylthiohexan-2-ol with formaldehyde and secondary amines. The structure of the synthesized compounds has been established by IR and (1)H NMR spectroscopy and mass spectrometry. The compounds have been tested as antimicrobial additives for lubricating oils and bacteria- and fungi-killing antiseptic agents. It has been found that they effectively suppress the vital activity of microorganisms.