The solubility of genistein in water, methanol, ethanol, propan-2-ol, 1-butanol, and ethyl acetate was measured at temperatures ranging from (280 to 333) K under atmospheric pressure using high-performance liquid chromatography (HPLC). Ethanol could be the most suitable solvent for purification and crystallization of geinstein. The experimental data were well-correlated with the Apelblat equation, which could be used as a useful model in the purification process of genistein.