Adhesion of uropathogens to epithelial cells is considered as an indispensable prerequisite for the manifestation of urinary tract infections. This study was carried out to investigate the influence of urinary constituents, such as glucose, lactose, urea and creatinine either alone or in combination, on the adhesion of uroisolates of Pseudomonas aeruginosa (planktonic and biofilm cells) to uroepithelial cells (UECs). It was observed that with increase in concentrations of these urinary constituents there was increase in adhesion of both cell forms of P. aeruginosa to UECs. This was true for all the strains of P. aeruginosa. The results of the present study bring out that environmental conditions prevalent in the host milieu under different physiological and pathological conditions have the potential to alter the adhesion ability of P. aeruginosa which may play an important role in deciding the ultimate outcome of an infection. (C) Koninklijke Brill NV, Leiden, 2009