화학공학소재연구정보센터

Protein Expression and Purification

Protein Expression and Purification, Vol.60, No.2 Entire volume, number list
ISSN: 1046-5928 (Print) 

In this Issue (21 articles)

97 - 102 Protein expression and purification of human Zbtb7A in Pichia pastoris via gene codon optimization and synthesis
Wang H, Wang QL, Zhang FF, Huang YH, Ji YL, Hou Y
103 - 109 Secretory expression of interferon-alpha 2b in recombinant Pichia pastoris using three different secretion signals
Ghosalkar A, Sahai V, Srivastava A
110 - 116 Frameshift events associated with the lysyl-tRNA and the rare arginine codon, AGA, in Escherichia coli: A case study involving the human Relaxin 2 protein
Kerrigan JJ, McNulty DE, Burns M, Allen KE, Tang X, Lu Q, Trulli JM, Johanson KO, Kane JF
117 - 126 Recombinant proprotein convertase 4 (PC4) from Leishmania tarentolae expression system: Purification, biochemical study and inhibitor design
Basak A, Shervani NJ, Mbikay M, Kolajova M
127 - 131 Expression and purification of active recombinant human bikunin in Pichia pastoris
Wang JQ, Yan FQ, Wang DD, Ban L, Sun N, Li CY, Zhang T, Yan WQ
132 - 139 Expression, purification and characterization of recombinant mouse translation initiation factor eIF4E as a dihydrofolate reductase (DHFR) fusion protein
Ghosh P, Cheng J, Chou TF, Jia Y, Avdulov S, Bitterman PB, Polunovsky VA, Wagner CR
140 - 146 An acidic and thermostable carboxymethyl cellulase from the yeast Cryptococcus sp S-2: Purification, characterization and improvement of its recombinant enzyme production by high cell-density fermentation of Pichia pastoris
Thongekkaew J, Ikeda H, Masaki K, Iefuji H
147 - 150 A rapid method for determining dynamic binding capacity of resins for the purification of proteins
Do T, Ho F, Heidecker B, Witte K, Chang L, Lerner L
151 - 156 Expression in bacteria of small and specific protein domains of two transcription factor isoforms, purification and monospecific polyclonal antibodies generation, by a two-step affinity chromatography procedure
Hernandez-Torres F, Pedrajas JR, Aranega AE, Navarro F
157 - 164 The 22.5 kDa spectrin-binding domain of ankyrinR binds spectrin with high affinity and changes the spectrin distribution in cells in vivo
Kolondra A, Grzybek M, Chorzalska A, Sikorski AF
165 - 169 High level expression of the light chain of botulinum neurotoxin serotype C1 and an efficient HPLC assay to monitor its proteolytic activity
Rawat R, Ahmed SA, Swaminathan S
170 - 175 Cloning, heterologous gene expression and biochemical characterization of the alpha-1,3-glucanase from the filamentous fungus Penicillium purpurogenum
Shalom G, Pratten J, Wilson M, Nair SP
176 - 181 Gram scale separation of casein proteins from whole casein on a Source 30Q anion-exchange resin column utilizing fast protein liquid chromatography (FPLC)
Plank J, Andres PR, Krause I, Winter C
182 - 187 High-level secretory expression, purification and characterization of Ailuropoda melanoleuca growth hormone in Pichia pastoris
Xu QG, Wu XC, Hou R, Liao MJ, Il KH, Shou JX, Bian HW, Han N, Pan JW, Zhang ZH, Zhu MY
188 - 193 Expression of frutalin, an alpha-D-galactose-binding jacalin-related lectin, in the yeast Pichia pastoris
Oliveira C, Felix W, Moreira RA, Teixeira JA, Domingues L
194 - 197 Bacterial expression and purification of Interleukin-2 Tyrosine kinase: Single step separation of the chaperonin impurity
Joseph RE, Andreotti AH
198 - 204 Cloning, E-coli overexpression, purification and binding properties of TraA and TraC, two proteins involved in the pheromone-dependent conjugation process in enterococci
Alfieri B, Folloni S, Elviri L, Gobbo M, Berni R, Folli C
205 - 213 Improved secretion of human Fas ligand extracellular domain by N-terminal part truncation in Pichia pastoris and preparation of the N-linked carbohydrate chain trimmed derivative
Muraki M
214 - 220 High-yield expression and purification of soluble forms of the anti-apoptotic Bcl-X-L and Bcl-2 as TolAIII-fusion proteins
Nedelkina S, Gokce I, Ridley H, Weckerle C, Magnin T, Vallette F, Pattus F, Lakey JH, Bechinger B
221 - 224 Expression, purification and characterization of pectin methylesterase inhibitor from kiwi fruit in Escherichia coli
Hao YL, Huang XY, Mei XH, Li RY, Zhai ZY, Yin S, Huang Y, Luo YB
225 - 231 Expression, purification, and initial characterization of human alanine aminotransferase (ALT) isoenzyme 1 and 2 in High-five insect cells
Liu L, Zhong S, Yang RZ, Hu H, Yu DZ, Zhu DL, Hua ZC, Shuldiner AR, Goldstein R, Reagan WJ, Gong DW