Protein Expression and Purification

Protein Expression and Purification, Vol.47, No.2 Entire volume, number list

ISSN: 1046-5928 (Print)

In this Issue (42 articles)

341 - 347	Cloning, expression, and purification of recombinant protein from a single synthetic multivalent construct of Mycobacterium tuberculosis Fang CM, Zainuddin ZF, Musa M, Thong KL
348 - 354	Conformation-specific affinity purification of proteins using engineered binding proteins: Application to the estrogen receptor Huang J, Koide A, Nettle KW, Greene GL, Koide S
355 - 366	High-throughput identification of refolding conditions for LXR beta without a functional assay Lin L, Seehra J, Stahl ML
367 - 373	The expression and refolding of isotopically labeled recombinant Matrilysin for NMR studies Ou L, Ma JB, Zheng XH, Chen X, Li GY, Wu HM
374 - 383	Expression and purification of sea raven type II antifreeze protein from Drosophila melanogaster S2 cells Scotter AJ, Kuntz DA, Saul M, Graham LA, Davies PL, Rose DR
384 - 392	Purification and characterization of the chaperone-like Hsp26 from Saccharomyces cerevisiae Ferreira RM, de Andrade LR, Dutra MB, de Souza MF, Paschoalin VMF, Silva JT
393 - 397	Expression and purification of two lipases from Yarrowia lipolytica AS 2.1216 Song HT, Jiang ZB, Ma LX
398 - 404	Gene synthesis, expression, purification, and characterization of human Jagged-1 intracellular region Popovic M, Coglievina M, Guarnaccia C, Verdone G, Esposito G, Pintar A, Pongor S
405 - 414	Purification, characterization, and mass spectrometric sequencing of a medium chain acyl-CoA synthetase from mouse liver mitochondria and comparisons with the homologues of rat and bovine Kasuya F, Tatsuki T, Ohta M, Kawai Y, Igarashi K
415 - 421	Constitutive expression of human pancreatic lipase-related protein 1 in Pichia pastoris Aloulou A, Grandval P, De Caro J, De Caro A, Carriere F
422 - 426	Enzymatic cleavage of fusion proteins using immobilised protease 3C Hedhammar M, Jung HR, Hober S
427 - 433	Expression, purification, and characterization of a novel recombinant fusion protein, rhTPO/SCF, in Escherichia coli Zang YH, Zhang X, Yuan DW, Zhang YM, Zhu J, Lu HQ, Chang C, Qin JC
434 - 440	Overproduction in Escherichia coli and purification of Epstein-Barr virus EBNA-1 Duellman SJ, Burgess RR
441 - 445	The Synthetic Gene Designer: A flexible web platform to explore sequence manipulation for heterologous expression Wu G, Bashir-Bello N, Freeland SJ
446 - 454	An expression vector tailored for large-scale, high-throughput purification of recombinant proteins Donnelly MI, Zhou M, Millard CS, Clancy S, Stols L, Eschenfeldt WH, Collart FR, Joachimiak A
455 - 460	Cloning, expression, and purification of the general stress protein YhbO from Escherichia coli Abdallah J, Kern R, Malki A, Eckey V, Richarme G
461 - 466	Expression, purification, and bioactivity of human tumstatin from Escherichia coli Gu QL, Zhang TY, Luo JX, Wang FY
467 - 476	Establishment of a yeast system that stably expresses human cytochrome P450 reductase: Application for the study of drug metabolism of cytochrome P450s in vitro Cheng J, Wan DF, Gu JR, Gong Y, Yang SL, Hao DC, Yang L
477 - 482	High-level expression of human stem cell factor fused with erythropoietin mimetic peptide in Escherichia coli Su L, Chen SS, Yang KG, Liu CZ, Zhang YL, Liang ZQ
483 - 489	Recombinant expression, purification, and functional analysis of two novel cystatins from sugarcane (Saccharum officinarum) Gianotti A, Rios WM, Soares-Costa A, Nogaroto V, Carmona AK, Oliva MLV, Andrade SS, Henrique-Silva F
490 - 497	Expression, purification, and refolding of the myeloid inhibitory receptor leukocyte immunoglobulin-like receptor-5 for structural and ligand identification studies Garner LI, Salim M, Mohammed F, Willcox BE
498 - 505	Cloning, expression, isotope labeling, and purification of human antimicrobial peptide LL-37 in Escherichia coli for NMR studies Li YF, Li X, Wang GS
506 - 515	High-level expression and purification of human thymidine kinase 1: Quaternary structure, stability, and kinetics Birringer MS, Perozzo R, Kut E, Stillhart C, Surber W, Scapozza L, Folkers G
516 - 523	Expression, purification, and characterization of His-tagged Staphylococcus xylosus lipase wild-type and its mutant Asp 290 Ala Mosbah H, Sayari A, Bezzine S, Gargouri Y
524 - 532	Overexpression and characterization of two human salivary proline rich proteins Pascal C, Bigey F, Ratomahenina R, Boze H, Moulin G, Sarni-Manchado P
533 - 541	Expression optimization and purification process development of an engineered soluble recombinant mouse linker of activation of T cells using surface enhanced laser desorption/ionization-mass spectrometry Brenac V, Mouz N, Schapman A, Ravault V
542 - 550	Purification and characterization of Mycobacterium tuberculosis 1D-myo-inosityl-2-acetamido-2-deoxy-alpha-D-glucopyranoside deacetylase, MshB, a mycothiol biosynthetic enzyme Newton GL, Ko M, Ta PL, Av-Gay Y, Fahey RC
551 - 561	Cloning, sequencing, expression, and characterization of protealysin, a novel neutral proteinase from Serratia proteamaculans representing a new group of thermolysin-like proteases with short N-terminal region of precursor Demidyuk IV, Kalashnikov AE, Gromova TY, Gasanov EV, Safina DR, Zabolotskaya MV, Rudenskaya GN, Kostrov SV
562 - 570	High efficiency single step production of expression plasmids from cDNA clones using the Flexi Vector cloning system Blommel PG, Martin PA, Wrobel RL, Steffen E, Fox BG
571 - 590	Molecular cloning and functional characterization of beta-N-acetylglucosaminidase genes from Sf9 cells Aumiller JJ, Hollister JR, Jarvis DL
591 - 598	BacMam recombinant baculovirus in transporter expression: A study of BCRP and OATP1B1 Hassan NJ, Pountney DJ, Ellis C, Mossakowska DE
599 - 606	Highly efficient expression and purification system of small-size protein domains in Escherichia coli for biochemical characterization Bao WJ, Gao YG, Chang YG, Zhang TY, Lin XJ, Yan XZ, Hu HY
607 - 613	Cloning, overexpression, purification, and characterization of O-acetylserine sulfhydrylase-B from Escherichia coli Zhao C, Kumada Y, Imanaka H, Imamura K, Nakanishi K
614 - 620	Expression, purification, and circular dichroism analysis of human CDK9 Leopoldino AM, Canduri F, Cabral H, Junqueira M, de Marqui ABT, Apponi LH, da Fonseca IO, Domont GB, Santos DS, Valentini S, Bonilla-Rodriguez GO, Fossey MA, de Azevedo WF, Tajara EH
621 - 628	Bacterial fermentation of recombinant major wasp allergen Antigen 5 using oxygen limiting growth conditions improves yield and quality of inclusion bodies Kischnick S, Weber B, Verdino P, Keller W, Sanders EA, Anspach FB, Fiebig H, Cromwell O, Suck R
629 - 633	Expression and purification of Pseudomonas aeruginosa SecA N-terminal domain: Stimulation of ATPase activity of the SecAL43P mutant protein Yu LY, Yang HC, Tai PC
634 - 639	Over-expression, purification, and characterization of aminopeptidase N from Escherichia coli Golich FC, Han M, Crowder MW
640 - 644	Production of recombinant Conkunitzin-S1 in Escherichia coli Bayrhuber M, Graf R, Ferber M, Zweckstetter M, Imperial J, Garrett JE, Olivera BM, Terlau H, Becker S
645 - 650	High-level expression and purification of Escherichia coli oligopeptidase B Yan JB, Wang GQ, Du P, Zhu DX, Wang MW, Jiang XY
651 - 661	Purification and characterization of the CK2 alpha'-based holoenzyme, an isozyme of CK2 alpha: A comparative analysis Olsen BB, Boldyreff B, Niefind K, Issinger OG
662 - 671	A novel fusion protein system for the production of native human pepsinogen in the bacterial periplasm Malik A, Rudolph R, Sohling B
672 - 681	Cloning, overexpression, and characterization of a thermoactive nitrilase from the hyperthermophilic archaeon Pyrococcus abyssi Mueller P, Egorova K, Vorgias CE, Boutou E, Trauthwein H, Verseck S, Antranikian G