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Expression of enzymes for 3 '-phosphoadenosine-5 '-phosphosulfate (PAPS) biosynthesis and their preparation for PAPS synthesis and regeneration Datta P, Fu L, He WQ, Koffas MAG, Dordick JS, Linhardt RJ Applied Microbiology and Biotechnology, 104(16), 7067, 2020 |
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Improved two-stage protein expression and purification via autoinduction of both autolysis and auto DNA/RNA hydrolysis conferred by phage lysozyme and DNA/RNA endonuclease Menacho-Melgar R, Moreb EA, Efromson JP, Yang T, Hennigan JN, Wang RX, Lynch MD Biotechnology and Bioengineering, 117(9), 2852, 2020 |
3 |
Scalable, two-stage, autoinduction of recombinant protein expression in E. coli utilizing phosphate depletion Menacho-Melgar R, Ye ZX, Moreb EA, Yang T, Efromson JP, Decker JS, Wang RX, Lynch MD Biotechnology and Bioengineering, 117(9), 2715, 2020 |
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Online Measurement of the Respiratory Activity in Shake Flasks Enables the Identification of Cultivation Phases and Patterns Indicating Recombinant Protein Production in Various Escherichia coli Host Strains Ihling N, Bittner N, Diederichs S, Schelden M, Korona A, Hofler GT, Fulton A, Jaeger KE, Honda K, Ohtake H, Buchs J Biotechnology Progress, 34(2), 315, 2018 |
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Highly efficient recombinant production and purification of streptococcal cysteine protease streptopain with increased enzymatic activity Lane MD, Seelig B Protein Expression and Purification, 121, 66, 2016 |
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A simplified protocol for high-yield expression and purification of bacterial topoisomerase I Jones JA, Price E, Miller D, Hevener KE Protein Expression and Purification, 124, 32, 2016 |
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Expression and purification of polioviral proteins in E. coli, and production of antisera as reagents for immunological assays Uma M, Rao PP, Nagalekshmi K, Hegde NR Protein Expression and Purification, 128, 115, 2016 |
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Lactose autoinduction with enzymatic glucose release: Characterization of the cultivation system in bioreactor Mayer S, Junne S, Ukkonen K, Glazyrina J, Glauche F, Neubauer P, Vasala A Protein Expression and Purification, 94, 67, 2014 |
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Use of slow glucose feeding as supporting carbon source in lactose autoinduction medium improves the robustness of protein expression at different aeration conditions Ukkonen K, Mayer S, Vasala A, Neubauer P Protein Expression and Purification, 91(2), 147, 2013 |
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Predictive tool for recombinant protein production in Escherichia coli Shake-Flask cultures using an on-line monitoring system Kunze M, Huber R, Gutjahr C, Mullner S, Buchs J Biotechnology Progress, 28(1), 103, 2012 |