| 초록 |
In tissue engineering, it is imperative to design scaffolds for cellular infiltration. To facilitate cellular response to the synthetic scaffold, we have developed a novel substrate mimicking structural and chemical properties of extracellular matrix using an electrospinning method and RGD peptide. Poly (L-lactide) (PLLA) terminated with carboxylic acid and poly (ethylene-glycol) (PEG)-diamine were reacted with EDC/NHS. The properties of PLLA-PEG were confirmed using NMR, ATR, and ESCA. Nanofiber mesh was fabricated using mixed solutions of PLLA and aminated PLLA. RGD peptide was then grafted using SPDP. To evaluate cellular response, MC3T3 cells were cultured onto the mesh. The investigation of the adherent cells on RGD-grafted meshes showed higher adhesion and well-spread morphology than non-treated groups. These results indicate that the RGD peptide promotes cellular response on the synthetic material, and electrospinning method provides a nano-scale environment for cell growth. |
