| 초록 |
Adsorbent treatment is an efficient method to remove waxy and tar compounds from plant cell cultures. In this study, sylopute, active clay (F-1), silica (SiO2), and magnesium oxide (MgO) were applied for the purification of the paclitaxel from plant cell cultures. There was selective adsorption of impurities in the treatment of sylopute and SiO2. Therefore, higher purity paclitaxel could be obtained simply in the following process with a higher yield than with active clay or MgO. The purities of crude paclitaxel after sylopute and SiO2 treatment followed by precipitation were increased up to 57.3% and 59.8%, while the purities for active clay and MgO were 46.9% and 48.4%, respectively. The overall yields of sylopute, SiO2 , active clay, and MgO were 77.3%, 94.2%, 49.1%, and 57.1%, respectively. The best result in removing impurities was obtained from the combination of sylopute or SiO2 treatment followed by precipitation process. Consequently, sylopute and SiO2 can be applied for production scale purification of other natural products as an alternative to synthetic adsorbents other alternative processes. |
