| 초록 |
We investigated thermosensitive liposomes which release their contents the physiological temperature and then we designed thermosensitive liposomes modified with changing of phospholipid ratio and (N-isopropylacrylamide/acrylamide) Copolymer. Therefore, We approached two problems; one is a change of phospholipid formulation, the other is a modification of thermosensitive liposome. In the first procedure, we prepared liposomes using the phospholipid without copolymers, and then we observed release of liposome. There were two liposome formulations prepared: TSL1, and TSL2. The TSL1 formulations were composed of DPPC:HSPC:CHOL:DSPE-PEG-2000(100:50:30:6). TSL2 formulations were DPPC:CHOL:DSPE-PEG-2000(100:30:6). In the second experiment, (N-isopropylacrylamide/acrylamide) Copolymer, which exhibit a lower critical solution temperature(LCST) in the physiological temperature, were prepared by free radical copolymerization. The copolymers were coated doxorubicin-loaded thermosensitive liposomes. Release of doxorubicin from these liposomes was studied with the change of temperature and time. We obtained that the release of thermosensitive liposome was based on the lipid formulation and ratio of copolymer. These copolymer-coated liposomes were promoted around and above the LCST of the copolymer.
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