| 초록 |
Calcium phosphate (CAP) has a wide range of applications in biomedical systems. Although there is great potential for the use of CAP in the development of gene delivery systems, the uncontrollable growth of CAP crystal makes it difficult to use in a practical nano-gene delivery system. In this study, we synthesized dopa (3,4-dihydroxy-L-phenylalanine) conjugated chitosan facilitating CAP particle stabilizer and gene carrier. The CAP nano-aggregates (CAP/pDNA/dopa-Chi) were successfully prepared by serial addition of plasmid DNA (pDNA) and dopa (3,4-dihydroxy-L-phenylalanine) modified chitosan to growing CAP particles. The addition of the dopa moiety is thought to enable chitosan adsorption onto the surface of forming calcium phosphate particles to prevent further growth. The dopa-Chi conjugate-stabilized CAP showed excellent biocompatibility and improvement of transfection efficiency, which should be of great potential to clinical studies as an alternative non-viral carrier. |
