| 초록 |
Despite enormous advances have been performed since the past decades, the lower mechanical strength, thrombosis, improper endothelialization associated issues are still found in small-diameter hybrid grafts. The study objective is to fabricate a dual layered biomaterials as a small-diameter vascular graft (＜2mm diameter) through electrospinning technique that mechanically and biologically matches the gold standard of blood vessel substitution. The presented graft will be composed of polycaprolactone/gelatin (PCL/Gel) which acts as an inner layer whereas polycaprolactone/Polyurethane (PUPCL) will be incorporated as the outer layer. To find out the optimum inner and outer layer, different concentration of natural and synthetic polymer combination fibrous membranes will be physico-mechanically characterized for surface structure, fiber diameter distribution, hydrophilicity, mechanical strength and degradation profile. To determine layers’s biocompatibility, outer layer will be seeded with fibroblast cells (L929) and inner layer will be treated with cow pulmonary endothelial cells (CPAE). After optimizing inner and outer layers, the developed electrospun small diameter graft will be further considered for detailed in vitro and in vivo experimental procedural steps with logical analysis to confirm its compliance. In conclusion, this antithrombogenic, mechanically strong small diameter engineered graft prepared using combinations of natural and synthetic polymers could modulate microstructure, porosity as well as typical cellular functions via migration, differentiation, required gene expression therefore could play a pivotal role in arena of vascular tissue regeneration application. |
