| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
기능성 고분자 |
| 제목 |
The specific binding interaction between poly(3-hydroxybutyrate) and the substrate binding domain of poly(3-hydroxybutyrate) depolymerase probed by two-dimensional infrared correlation spectroscopy |
| 초록 |
We examined the specific binding interaction between the substrate binding domain (SBD) of poly(3-hydroxybutyrate) (PHB) depolymerase and PHB using two-dimensional infrared correlation spectroscopy. Some of the amino acid residues in the SBD are expected to contribute to the specific biding of the SBD on the surface of PHB. Positively charged histidine and arginine residues seem to have electrostatic interaction with the carbonyl group of PHB, and asparagines and glutamine with polar side chains are also suggested to be involved in the molecular recognition between the SBD and PHB. We measured the infrared spectra of the PHB film in the presence of SBD solution at different humidity. The cross-peaks in the 2D synchronous correlation spectra indicate that the amino acid residues with hydrophilic side chains have interactions with carbonyl groups in the PHB. |
| 저자 |
박원민1, 박태정1, 정영미2, 박호석1, 홍원희1, 이상엽1
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| 소속 |
1한국과학기술원, 2강원대 |
| 키워드 |
PHB; SBD; PHB depolymerase; specific binding
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| E-Mail |
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