| 학회 |
한국고분자학회 |
| 학술대회 |
2007년 봄 (04/12 ~ 04/13, 제주 ICC) |
| 권호 |
32권 1호 |
| 발표분야 |
나노바이오기술용 고분자(의료용 고분자 부문위원회) |
| 제목 |
Gold Quenched Near-Infrared Fluorescence Imaging Probe |
| 초록 |
The optical imaging probe for cancers was successfully developed based on NIRF fluorophore (Cy5.5) labeled MMP-2 substrate on the surface of gold nanoparticles. Super quenching effect of Cy5.5 fluorescence on the surface of gold nanoparticles was gained by fluorescence resonance energy transfer between closely located Cy5.5 fluorophores as well as by surface resonance plasmon effect of gold nanoparticles. After the activation by the presence of MMP-2, however, the peptide connection between gold nanoparticles and Cy5.5 was selectively cleaved and Cy5.5 became fluorescent. The probe recovered strong fluorescent signals after the enzymatic degradation in vitro and in vivo. Considering all the fact, the proposed optical probes are potentially excellent candidates for the field of enzyme activity assay in vitro and in vivo imaging of tumors. |
| 저자 |
차의준1, 이슬기2, 박경순2, 권익찬2, 안철희1
|
| 소속 |
1서울대, 2KIST |
| 키워드 |
gold nanoparticles; NIRF; protease; enzyme; quenching; imaging
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| E-Mail |
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