| 초록 |
Measuring the amounts of free amino acid (FAA) has been needed in several areas. Current methods to measure the amounts of FAAs is colorimetric assay using Nynhydrin and chromatography, but the Nynhydrin assay has different results depending on the species of FAA. For accurate analysis of FAA contents, it is almost the only way to measure and integrate the amount of each FAA by chromatography. However, chromatography is difficult to use in the field. To this end, a carbon paper (CP) electrode modified with L-amino acid oxidase (LAAO) was designed. The LAAO breaks down all kinds of FAA except Proline to produce hydrogen peroxide (HP). CP electrode for detecting HP has been functionalized with manganese oxide that can specifically detect HP. Therefore, the developed sensor replaces FAA with HP regardless of species through LAAO attached to the sensor and measures total FAA contents with a signal through HP. |
