| 학회 |
한국재료학회 |
| 학술대회 |
2018년 봄 (05/16 ~ 05/18, 삼척 쏠비치 호텔&리조트) |
| 권호 |
24권 1호 |
| 발표분야 |
B. 나노화학/바이오 분과 |
| 제목 |
Enzymatically cross-linked injectable chitosan/gelatin/BCP based hydrogel as a growth factor carrier for bone tissue engineering |
| 초록 |
The goal of this study is the development of a novel injectable system which is in situ forming biomimetic bone filler consisted of the modified gelatin and chitosan hydrogel incorporating with bi-calcium phosphate (BCP) and bone morphogenetic protein-2 (BMP-2). The system mainly consists of tyramine-conjugated gelatin (GT) and phenolic hydroxyl group-conjugated chitosan (CP), which can be formed to gel phase via enzymatic reaction using horseradish peroxidase (HRP) and hydrogen peroxide (H2O2) under physiological conditions. To improve the capabilities of osteogenesis, BCP nanoparticles and BMP-2 were loaded into this system. The CP-GT with BCP/BMP-2 hydrogel was characterized by scanning electron microscopy, electron dispersion spectroscopy, and X-ray diffraction. The hydrogel structure showed a porous interconnected morphology. Biocompatibility of this hydrogel was evaluated using MC3T3-E1 via WST assay and F-actin staining. The hydrogel was maintained in viscous phase after injection with cells, showed suitable degradation rate and improved cell viability and proliferation. These results highlight the potential of using a novel injectable biomimetic scaffold for prevention of bone disease. |
| 저자 |
Boram Kim1, Mirana Taz2, Byong-Taek Lee3
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| 소속 |
1Institute of Tissue Regeneration, 2College of Medicine, 3Soonchunhyang Univ. |
| 키워드 |
Injectable hydrogel; Bone filler; Chitosan; Gelatin; BCP; Growth factor
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| E-Mail |
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