| 학회 | 한국화학공학회 |
| 학술대회 | 2017년 가을 (10/25 ~ 10/27, 대전컨벤션센터) |
| 권호 | 23권 2호, p.1751 |
| 발표분야 | 생물화공 |
| 제목 | Parallel Structural dsDNA/Recombinant Azurin hybrid as DNA Conductance Booster for miRNA and viral DNA Detection |
| 초록 | Here, we firstly reported a novel hybrid structure consists of parallel structural dsDNA (PSD) conjugated with recombinant azurin (rAzu) to increase the dsDNA electrical conductivity and control the charge transport (CT) migration. Then, this novel hybrid structure was applied for electrochemical detection of nucleic acids with ultra-sensitivity towards single base pair mutation. DNA conductivity was measured based on the reduction of Ag+ ion intercalated between C-C mismatches of dsDNAs at the top of each structure. The CT mechanism across the hybrid structure was clarified on the basis of the redox potential location of the molecules. The proposed PSD/rAzu hybrid with the ability of miRNA expression level profiling in cancer cells can be used as a general platform for precise detection of viral DNAs and RNAs. Acknowledgments: This research was supported by Basic Science Research Program through the National Research Foundation of Korea (NRF) funded by the Ministry of Education (2016R1A6A1A03012845). |
| 저자 | 모하마드니아이 모센1, 이 택2, 윤진호1, 최정우1 |
| 소속 | 1서강대, 2광운대 |
| 키워드 | 생물화공 |
| 원문파일 | 초록 보기 |
