| 초록 |
Histamine is one of the important organic compounds, but it is well known factor to cause an allergy and inflammation-like food poisoning. Therefore, detection of the histamine in food is an important issue for maintaining food safety. In this study, we successfully detect the histamine using lateral flow assay (LFA) which is a rapid, sensitive, cost-effectiveness and highly popular point-of-care (POC) technique. The assay used a specific antibody for histamine as receptor and gold nanoparticles as probe for detection of histamine in fish through competitive LFA strips. With this method, 50 ppm of histamine can be detected in less than 10 min in fish with simple pretreatment of samples. Moreover, Specificity was also evaluated against histamine and histamine analogues. We expect that our LFA sensor was significantly faster than the conventional analysis such as HPLC, ELISA, GC/MS, etc. and possesses the potential for rapid POC testing for food safety monitoring of histamine. |
