| 학회 |
한국고분자학회 |
| 학술대회 |
2015년 가을 (10/06 ~ 10/08, 대구컨벤션센터(EXCO)) |
| 권호 |
40권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 II |
| 제목 |
Differentiation capacity of human mesenchymal stem cell into discogenic phenotype using co-culture system in alginate hydrogel with PCL nanofibers |
| 초록 |
Intervertebral disc (IVD) degeneration is associated with increased cell senescence and apoptosis. For the 3D culture of tissue engineering, hydrogel and mesh structures are widely used. Using electrospinning methods, we fabricated PCL nanofiber for mesh structures and using alginate, hydrogel was fabricated. For the cellular approaches of disc tissue engineering, human mesenchymal stem cells (hMSCs) provide plausible cell source and cell co-culture system of hMSCs and IVD cells is proved for notable efficacy in cell differentiation. We first seeded hMSCs onto PCL nanofibers. After attachment, IVD cells were seeded into alginate hydrogel which encapsulating PCL nanofibers. Each hMSCs and IVD cells were seeded on PCL nanofibers and in alginate hydrogel as control. Our study suggests that the hMSCs can be differentiated into discogenic phenotype by co-culture, which provides excellent mechanism for hMSCs based tissue engineering for IVD regeneration. |
| 저자 |
홍혜진, 조강희, 차성호, 윤병주, 고원건, 국윤민
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| 소속 |
연세대 |
| 키워드 |
Intervertebral disc; mesenchymal stem cell; alginate; PCL; co-culture
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| E-Mail |
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