| 학회 |
한국고분자학회 |
| 학술대회 |
2014년 봄 (04/10 ~ 04/11, 대전 컨벤션센터) |
| 권호 |
39권 1호 |
| 발표분야 |
의료용 고분자 부문위원회(초청-영어발표/포스터-국문발표 가능) |
| 제목 |
Improved tissue adhesion of gelatin hydrogels based on a dual enzymatic crosslinking |
| 초록 |
Stable adhesion of materials to the surrounding tissue is considered important in improving in vivo performance of in situ forming hydrogels. Recently we have shown improved tissue adhesion of phenol-derivatized gelatin hyrogels formed via horseradish peroxidase (HRP)-catalyzed cross-linking. However, a limited number of tyrosine residues on tissue surfaces might impede maximizing the extent of phenolic coupling. In this study, we present a dual enzymatic crosslinking system based on HRP and tyrosinase to prepare gelatin hydrogels with improved tissue adhesion. The highest adhesive strength of 34 kPa was obtained using HRP-tyrosinase, which was almost a 2-fold higher than that of the hydrogel prepared with HRP (19 kPa). All hydrogels were completely degraded in the presence of collagenase and the encapsulated hDFBs were almost viable in the gel matrices. Therefore, we expect that the dual enzymatic crosslinking system can be useful for the development of tissue adhesive hydrogels. |
| 저자 |
풍, 배진우, Thai Thanh Hoang Thi, Dai Hai Nguyen, 이윤기, 박기동
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| 소속 |
아주대 |
| 키워드 |
Gelatin hydrogels; dual enzymatic cross-linking; tissue adhesive
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| E-Mail |
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