| 학회 | 한국화학공학회 |
| 학술대회 | 2004년 봄 (04/23 ~ 04/24, 공주대학교) |
| 권호 | 10권 1호, p.420 |
| 발표분야 | 생물화공 |
| 제목 | Optimization of Culture medium and Conditions for Lactosucrose Production by Sterigmatomyces elviae CBS 8119 Using Statistical Analysis |
| 초록 | Lactosucroses (4G-β-Galactosylsucrose) have attracted attention in response to an increasing demand for so-called health foods with functional properties such as calorie free, non-cariogenic, decreasing the total cholesterol and lipid in serum, the promotion of animal growth and improving the intestinal bifidobacterium. They are produced from sucrose and lactose by the action of β-fructofuranosidase (EC 3.2.1.26) producing from various microorganisms such as Arthrobacter sp, sprobomyces sp etc. The objective of this study is to optimize the culture medium and conditions for the production of lactosucrose by Sterigmatomyces elviae mutants. And continuous production using whole cell enzyme immobilization was carried out with aim of obtaining a high productivity of lactosucrose. Optimized media for the lactosucrose production using statistical experimental design and were determined to be 1.21 % sucrose, 0.98% yeast extract, 2.92% bactopeptone and 0.536% ammonium sulfate. Then, the carbon source and ammonium sulfate have an important effect on the production of lactosucrose. The optimized culture conditions were determined to be 10% inoculum size, pH 6.5 and 50℃. |
| 저자 | 이종호1, 임정수1, 박승원2, 김정훈2, 김승욱1 |
| 소속 | 1고려대, 2제일제당 |
| 키워드 | Optimization; Lactosucrose; Sterigmagomyces elviae; Statistical method |
| 원문파일 | 초록 보기 |
