| 초록 |
Brown macroalgae consists of alginate, mannitol and glucan. However, it is difficult for industrial microbes to use alginate due to the lack of utilization pathway. Thus, to convert brown macroalgae into valuable chemicals, its utilization pathway should be introduced and optimized for maximum yield and productivity. In this research, two types of strategies were used to solve these problems. First, In order to degrade alginate polysaccharide, MJ3 alginate lyase which can degrade alginate was overexpressed. Second, metabolic pathway was introduced to E. coli through the synthetic biology approach. The genes encoding alginate metabolic pathway were expressed under synthetic promoter and optimized 5’-UTR level. It is expected that the engineered strain will show capability to directly utilize alginate as carbon sources and this microbial platform will be further applied to produce fuels and chemicals. |
