| 학회 |
한국고분자학회 |
| 학술대회 |
2009년 가을 (10/08 ~ 10/09, 광주과학기술원 오룡관) |
| 권호 |
34권 2호 |
| 발표분야 |
약물 및 유전자 전달용 고분자(의료용 고분자 부문위원회) |
| 제목 |
Superparamagnetic Iron oxide nanoparticles(SPION)-embedded Supra-assembly-mediated gene delivery guided by enhanced Magnetic resonance(MR) signal |
| 초록 |
Recently, combination of SPION-based diagnostic properties with accompanying therapeutics such as drugs or genes has been explored to improve their therapeutic efficacy. In this regard, positively charged SPION-loaded polymersome tethering PEG as a hydrophilic corona layer was prepared to deliver therapeutic genes to target sites, which was monitored by MRI concomitantly. Complete nanoparticle formation of SPION-loaded polymersome with plasmid DNA was checked. Hydrodynamic particle size of SPION-embedded supra-assembly was found to range between 110 and 160nm, and its surface charge was slightly positive confirmed by zeta potential measurement. The transfection efficiency in vitro was tested by treating 4T1 breast cancer cell line with luciferase plasmids/SPION-embedded supra-assembly complex. The detectability of SPION in transfected cells by MRI was also checked. This result indicates that the SPION-embedded supra-assembly can be applied to MR imaging guided gene therapy. |
| 저자 |
이상준1, Hieu Quang Vu1, 이현진2, Y.T.C. Nguyen1, Hui-Lian Che1, 정용연1, 박인규1
|
| 소속 |
1전남대, 2과학기술원 생명화학공학부 |
| 키워드 |
MRI; gene therapy
|
| E-Mail |
|
