| 학회 |
한국공업화학회 |
| 학술대회 |
2021년 가을 (11/03 ~ 11/05, 대구 엑스코(EXCO)) |
| 권호 |
25권 2호 |
| 발표분야 |
포스터-생물공학 |
| 제목 |
Kinetic separation in Escherichia coli by enzyme engineering for itaconate production |
| 초록 |
Microbial production of itaconate has been mainly conducted by Aspergillus terreus. Recently, its production in Escherichia coli has been researched due to the complicated fermentation process and shortage of genetic engineering tools of A. terreus. Unilke A. terreus, spatial separation of metabolic flux for the itaconate production has been hardly feasible in E. coli, which leads to the low level of its precursor, cis-aconitate. In this study, directed evolution of protein known to have substrate promiscuity to citrate was conducted in E. coli to enhance the cis-aconitate availability with the help of itaconate responsive screening system. Thereafter, the developed mutant showed significantly enhanced catalytic efficiency to citrate compared to the wild-type enzyme and consequently, its use enabled significantly increased itaconate production compared to the parental strain, indicating the successful enhancement of cis-aconitate supply by kinetic separation |
| 저자 |
예대열1, 노명현2, 문조현1, 이정욱1, 양재성3, 정규열1
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| 소속 |
1포항공과대, 2UCSD, 3CRAG |
| 키워드 |
Metabolic engineering; Itaconate; Kinetic separation; Enzyme engineering
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| E-Mail |
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