| 학회 |
한국고분자학회 |
| 학술대회 |
2017년 가을 (10/11 ~ 10/13, 제주컨벤션센터) |
| 권호 |
42권 2호 |
| 발표분야 |
의료용 고분자 부문위원회 I |
| 제목 |
Dual Trigger responsive albumin-polyplex nanocomplex for enhanced gene delivery in breast cancer |
| 초록 |
Trigger responsive gene transporters play a pivotal role in enhancing transfection efficiency with minimal cell toxicity. In the current study, a dual trigger controlled gene releasing nanocomplex was developed by complexing peptide conjugated cationic polyplex with NIR dye loaded albumin. It formed a stable complex with DNA and exhibited high gene transfection efficiency without any sign of cytotoxicity in breast cancer cells. Moreover, it demonstrated an enhanced gene transfection efficiency upon NIR laser irradiation. To investigate the endosomal escaping effect and redox triggered gene release of the nanocomplex, proton sponge and glutathione inhibitors were employed and the endosomal escape mediated by the local heat generated by nanocomplex upon irradiation and redox mediated gene release from the polyplex was confirmed. With addition of cell penetrating peptide to the nanocomplex, tumor penetration was achieved in tumor spheroid model. |
| 저자 |
Rajendrakumar Santhosh Kalash1, 박인규2
|
| 소속 |
1Chonnam National Univ. Medical School, 2Chonnam National Univ. |
| 키워드 |
Photothermal; gene delivery; Bovine serum albumin; IR780; cancer.
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| E-Mail |
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