| 학회 |
한국고분자학회 |
| 학술대회 |
2013년 봄 (04/11 ~ 04/12, 대전컨벤션센터) |
| 권호 |
38권 1호 |
| 발표분야 |
의료용 고분자 부문위원회 |
| 제목 |
Microneedle-based polyplex delivery system for DNA vaccine: potential use for Alzheimer’s disease |
| 초록 |
In this study, microneedle-based gene delivery system which allows triggered release of DNA/polymer polyplexes upon the pH changes was developed using the layer-by-layer assembly of multilayered polyelectrolytes composed of negatively charged heparin and cationized albumin which performs pH-dependent charge-inversion. Mannosylate polyethylenimine-deoxycholic acid conjugate was synthesized and used as a non-toxic cationic carrier for polyplex with plasmid DNA and targeting gene delivery to resident macrophages in subcutaneum. The polyplexes was placed onto the multilayered microneedles as an outer most layer via electrostatic interactions. The pH transition to neutral pH allows accelerated release of polyplexes in vitro. Immunization of plasmid DNA encoding amyloid beta monomer(Aβ1-42) using the multilayered microneedle system demonstrated superior antigen expression and antibody (anti-Aβ1-42 IgG) production in vivo, compared to conventional subcutaneous injection with syringe. |
| 저자 |
김낙원, 이규리, 이민상, 정지훈
|
| 소속 |
성균관대 |
| 키워드 |
DNA Vaccine; Microneedle; Polyplex; Macrophage targeting
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| E-Mail |
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