| 학회 |
한국공업화학회 |
| 학술대회 |
2015년 가을 (11/04 ~ 11/06, 제주국제컨벤션센터(ICCJEJU)) |
| 권호 |
19권 2호 |
| 발표분야 |
나노_포스터 |
| 제목 |
Highly Fluorescent Graphene Oxide Biosensors for Determination of AChE Activity Involved in Alzheimer`s Disease |
| 초록 |
Alzheimer`s disease is caused by the self-assembling of amyloid beta peptides producing fibril and their accumulation in the brain, which is known to be accelerated by acetylcholinesterase (AChE) catalyzing the hydrolysis of acetylcholine (ACh) to give choline and acetic acid. Herein, we demonstrate a simple approach for designing a graphene oxide (GO) fluorescence-based biosensor for the detection of AChE activity and inhibitors. GO was non-covalently functionalized with phenoxy-modified dextran polymer (PhO-dex-GO), leading to significant improvement of its colloidal and optical stability in various media solutions. The fluorescence of PhO-dex-GO increased as acetylthiocholine (ATCh) was hydrolyzed by AChE, but no fluorescence increase was observed without the enzymatic hydrolysis reaction. Based on this turn-on fluorescence response, the PhO-dex-GO sensor was able to report kinetic and thermodynamic parameters in relation to AChE activity and inhibition. |
| 저자 |
주종민, 이혜림, 임다빈, 박정현, 김혜인, 전수지, 김종호, 강태욱
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| 소속 |
한양대 |
| 키워드 |
graphene oxide; optical biosensor; GO fluorescence; Alzheimer`s disease; enzyme activity detection
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| E-Mail |
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