| 초록 |
Irreversibility of the atherogenic cascades highlight the need for early diagnosis and prevention. Disturbed blood flow is one of the earliest atherogenic events, inducing endothelial dysfunction and subsequent inflammatory responses. Here, we present a human mesenchymal stem cell (hMSC)-derived nanovesicle (NV) with the peptide GSPREYTSYMPH (PREY) (PMSC-NVs) that can target disturbed flow sites. The PMSC-NVs were effectively produced from hMSCs using plasmid DNA designed to functionalize the cell membrane by displaying PREY on the outer surface. We confirmed the potent therapeutic and diagnostic effect using in vivo mouse and porcine partial carotid artery ligation model or human microfluidic disturbed flow model, suggesting the potential for clinical translation by using models from multiple species. This nanoscale platform is expected to contribute to the development of new theragnostic strategies for preventing the progression of atherosclerosis. |
