| 초록 |
In this study, we utilized a boronic acid functionalized fluorescent carbon dot (FCD), which has small size, excellent photoluminescent properties, and high biocompatibility. Next, FCD was conjugated with a fluorescent probe (DYE) that caused quenching via electron-receiving ability through the Förster resonance energy transfer (FRET) phenomenon. Here, FCD-DYE could selectively bind to diol group of the bacterial cell surface by forming boronate ester bond that can be used for the detection of bacteria. In the presence of bacteria, DYE is released from FCD, replaced by diol groups on bacteria cell via generating new cyclic ester bonds with FCD, resulting in fluorescent recovery. The system is not only relatively inexpensive and simple, but also become a powerful biosensor that exhibits high sensitivity and affinity to detect bacteria. This work was supported by Korea National University of Transportation in 2019. |
