| 학회 |
한국공업화학회 |
| 학술대회 |
2018년 봄 (05/02 ~ 05/04, 대구 엑스코(EXCO)) |
| 권호 |
22권 1호 |
| 발표분야 |
생체재료_포스터 |
| 제목 |
Comparison of chemical and physical cross-linked three-dimensional gelatin scaffold |
| 초록 |
The bioink is one of the most important factors in three-dimensional (3D) bioprinting. Especially, natural polymers such as gelatin having high biocompatibility are attractive materials. However, gelatin has poor mechanical properties and cross-linking is required to maintain the 3D structure. N-Hydroxysuccinimide (NHS) is widely used for gelatin cross-linking, but residual NHS can be toxic to cells. In this study, physical cross-linking via dried heat treatment (DHT) was used to reduce cytotoxicity caused by chemical cross-linking. We fabricated 3D scaffold using 3D bioprinting and cross-linked by DHT. We used a SEM to determine the surface morphology of 3D scaffold and chemically bond exchange was confirmed by FT-IR. For evaluating the biological effect on 3D scaffold cross-linked by DHT, human dermal fibroblast was cultured on 3D scaffold. The DNA assay shows that the cell proliferation of DHT scaffold was 44% higher than NHS cross-linked 3D scaffold after 14 days of culture. |
| 저자 |
최동진1, 박상준2, 고영재2, 김영진3, 정석4, 김천호2
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| 소속 |
1한국원자력의학원 / 고려대, 2한국원자력의학원, 3대구가톨릭대, 4고려대 |
| 키워드 |
3D bioprinting; gelatin; 3D scaffold
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| E-Mail |
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