| 학회 |
한국고분자학회 |
| 학술대회 |
2008년 봄 (04/10 ~ 04/11, 컨벤션 뷰로(대전)) |
| 권호 |
33권 1호 |
| 발표분야 |
의료용 고분자 |
| 제목 |
EGFP Transfected Rat Pheochromocytoma PC-12 Cells Using PEI. |
| 초록 |
PC12 cell-based gene therapy is promising for the reconstruction of injured tissues or organs. Thus, the aim of this study is to evaluate transfection efficiency in PC12 cell using DNA/polyethyleneimine (PEI) complexes. The formation of complexes from DNA and PEI was performed by the adding of the PEI solution to the DNA solution. The particle size of DNA/PEI complexes measured by dynamic light scattering (DLS) showed diameters of up to 500 nm. These results indicated that DNA was sufficiently condensed by PEI to give DNA/PEI complexes. The viability of PC12 cell in the presence of DNA/PEI complexes was evaluated by the MTT assay. After gene transfection, enhanced green fluorescence protein (EGFP) was monitored by flow cytometry. Naked DNA showed no EGFP expression, whereas delivery of DNA/PEI complexes to PC12 cell showed EGFP expression and resulted in 0-14% transfection. In conclusion, we have shown that PEI acts as an effective gene carrier for PC12 cell |
| 저자 |
이정화1, 안현희1, 김경숙1, 이주영1, 김문석1, 이봉2, 강길선3, 이해방1
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| 소속 |
1한국화학(연), 2부경대, 3전북대 |
| 키워드 |
rat pheochromocytoma PC-12 cells; gene delivery; nonviral; PEI; lipofectamine
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| E-Mail |
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