| 학회 |
한국화학공학회 |
| 학술대회 |
2007년 가을 (10/26 ~ 10/27, 한국과학기술원) |
| 권호 |
13권 2호, p.1738 |
| 발표분야 |
생물화공 |
| 제목 |
Analysis of Toxic Effects of Silver Nanoparticles on Escherichia coli Using Bioluminescence Bacteria |
| 초록 |
This study shows that the toxic effects of actions by AgNPs were compared with the results obtained from AuNPs and silver nitrate, using the stress promoter responses from eight bioluminescent bacteria. Based on the changes in the bioluminescence, the AgNPs resulted in a higher cellular toxicity than the same amount of AuNPs. In addition, the AgNPs cause heat shock and oxidative damage to the bacterial cells, but not DNA damage, while AuNPs had no response at the same test concentrations. Using two enzymes, superoxide dismutase and catalase, which relieved oxidative stress, added to them with the AgNPs showed a reduced induction of the sodA promoter when compared to cultures exposed to AgNPs alone, indicating that oxidative radicals are being generated due to the nanoparticles. Silver nitrate, used to evaluate differences between the silver ion and the nano-sized silver particles, was also found to causes heat shock damage and oxidative stress within the bacterial cells. However, induction of silver nitrate was not relieved by the addition of enzymes, indicating that there is difference in toxic effects of action between the silver nanoparticles and the silver ion. |
| 저자 |
채윤주1, 황이택1, 이진형2, 김연석1, 김병찬3, 상병인4, 구만복1
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| 소속 |
1고려대, 2GIST, 3파스퇴르(연), 4KIST |
| 키워드 |
Nanoparticle toxicity; Recombinant bioluminescent bacteria; Silver nanoparticle; toxic mechanism |
| E-Mail |
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| 원문파일 |
초록 보기 |
