| 학회 | 한국고분자학회 |
| 학술대회 | 2005년 가을 (10/13 ~ 10/14, 제주 ICC) |
| 권호 | 30권 2호 |
| 발표분야 | 의료용 고분자 부문위원회 |
| 제목 | In Vitro and In Vivo Degradation Behaviors of Acetylated Chitosan Porous Beads |
| 초록 | Chitosans are non-toxic and biocompatible cationic natural polymers which are composed of β-1,4-linked D-glucosamine (GlcN) and N-acetyl-D-glucosamine (GlcNAc) units with various compositions.1 The degree of acetylation (DA) represents the portion of GlcNAc units with respect to total number of units. The DA influences not only the biodegradability, but also the biocompatibility of chitosans.2 In our previous study, we synthesized DA-controlled chitosans (DA values from 10 to 90%) by the acetylation reaction of deacetylated chitosan (DA ∼ 10%) and acetic anhydride with different ratio to provide controllable degradation rate. In this study, we fabricated injectable porous beads (diameter ~500 μm) using the acetylated chitosans with different DA values (from 10 to 50%) and their in vitro and in vivo degradation behavior were investigated. In vitro degradation experiment was conducted in the solutions of lysozyme and N-acetyl-β-D-glucosaminidase (separately and both together), which are enzymes in the human body inducing chitosan degradation.3 The degradation behavior of the acetylated chitosan beads in the enzyme solutions was evaluated by the measurements of end group (UV), molecular weight (GPC) and weight loss (Fig. 1). The acetylated chitosan beads were also examined to evaluate their in vivo degradation behavior by subcutaneous implantation of the beads into the back of SD rats. The results were evaluated by the measurement of molecular weight (GPC) and histological examination (H&E staining). Fig. 1. In vitro degradation behavior of acetylated chitosan beads in different enzyme solutions; (a) lysozyme, (b) N-acetyl-β-D-glucosaminidase, and (c) lysozyme/N-acetyl-β-D-glucosaminidase mixture. Acknowledgment This work was supported by a grant from Korea Ministry of Commerce, Industry and Energy. References 1. A. Domard and M. Rinaudo, Int. J. Biol. Macromol., 5, 49, (1983). 2. T. Freier, H. S. Koh, K. Kazazian and M. S. Shoichet, Biomaterials, 26, 5872 (2005). 3. R. Muzzarelli, Polymeric Biomaterials, S. Dumitriu, Ed. 1994, pp 179. |
| 저자 | 송대근1, 오세행1, 이동신2, 배은희2, 이진호1 |
| 소속 | 1한남대, 2리젠바이오텍 |
| 키워드 | Acetylated chitosan; Porous bead; Biodegradability |
