| 학회 |
한국재료학회 |
| 학술대회 |
2011년 봄 (05/26 ~ 05/27, 제주 휘닉스 아일랜드) |
| 권호 |
17권 1호 |
| 발표분야 |
D. Health and Biological Materials(생체 및 의용재료) |
| 제목 |
In-vitro and in-vivo biocompatibility evaluation of silica based bio-active glass prepared by hydrothermal method |
| 초록 |
Bioactive glass powders were synthesized by hydrothermal chemical route by the use of ultrasonic energy irradiation. We used sodalime, calcium nitrate tetra hydrate and di ammonium hydrogen phosphate as the precursor material to synthesize SiO2 rich bio-active glass materials. The SiO2 content was varied in the precursor mixture to 60, 52 and 45 mole%. Dense compacts were obtained by microwave sintering at 1100 °C. Mechanical properties were characterized for the fabricated dense bioactive glasses and were found to be comparable with conventional CaO-SiO2-Na2O-P2O5 bioactive glass. Detailed biocompatibility evaluation of the glass composition was investigated by in-vitro culture of MG-63 cell and mesenchyme stem cell. Cell adhesion behavior was investigated for both of the cell by one cell morphology for 30, 60 and 90 minutes. Cell proliferation behavior was investigated by culturing both of the cells for 1, 3 and 7 days and was found to be excellent. Both SEM and confocal laser scanning microscopy were used for the investigation. Western blot analysis was performed to evaluate the bimolecular level interaction and extent and rate of specific protein expression. The ability to form biological apatite in physiological condition was observed with simulated body fluid (SBF). In-vivo bone formation behavior was investigated after implanting the materials inside rabbit femur for 1 and 3 month. The bone formation behavior was excellent in all the bioglass compositions, specially the composition with 60% SiO2 content showed most promising trend. |
| 저자 |
swapan kumar sarkar1, Phuong Thi Nguyen2, Byong Taek Lee1
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| 소속 |
1Soonchunhyang Univ., 2Soonchonhyang Univ. |
| 키워드 |
Bioactive glass; biocompatibility; hydrothermal method; In-vivo
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| E-Mail |
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