| 초록 |
In our previous report, Enterobacter aerogenes was engineered for glucose and xylose co-utilization and 2,3-butanediol production. Here, the strain EMY-22 was further engineered to improve the titer, productivity, and yield by reducing byproducts production. To reduce succinate production, the budABC operon and galP gene were overexpressed in the strain, which increased the 2,3-butanediol production. For further reduction of succinate and 2-ketogluconate production, maeA was also overexpressed. The engineered strain produced 2,3-butanediol with longer production duration and reduced byproduct formation from sugarcane bagasse hydrolysate in flask cultivation. The engineered strain showed improvements in titer, yield, productivity compared to its parental strain in fed-batch fermentation. This study proved that the metabolic engineering for reduced byproduct formation was a promising strategy to improve 2,3-butanediol fermentation using lignocellulosic biomass. |
