| 학회 |
한국공업화학회 |
| 학술대회 |
2017년 봄 (05/10 ~ 05/12, 광주 김대중컨벤센센터(Kimdaejung Convention Center)) |
| 권호 |
21권 1호 |
| 발표분야 |
생물공학 |
| 제목 |
Comparative and quantitative targeted glycomics using stable isotopic labeling (i-QTaG) |
| 초록 |
Here, as a proof-of-concept study, we developed a stable isotopic labeling-based quantitative targeted glycomics technique for the comparative and quantitative analysis of total N-glycans using matrix-assisted laser desorption/ionization time-of-flight mass spectrometry (MALDI-TOF MS). The intensities of the N-glycan peaks from quantification method showed a good linearity with the amount of the bovine fetuin glycoproteins. The ratios of intensity between the isotopically 2-AA labeled N-glycans were close to the theoretical molar ratios (1:1, 1:2, 1:5). This report demonstrated that our method has wide applications in many fields of clinical and biochemical engineering through developing 96 or 384-well plate-based i-QTaG procedures, which would be included in the following study. |
| 저자 |
안다희1, 황철환1, 박한규1, 송원석2, 정윤이1, 김성민1, 김윤곤1
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| 소속 |
1숭실대, 2서울대 |
| 키워드 |
N-glycan; MALDI-MS; stable isotopic labeling; comparative quantitation
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| E-Mail |
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