| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 봄 (04/26 ~ 04/28, ICC 제주) |
| 권호 |
23권 1호, p.508 |
| 발표분야 |
생물화공 |
| 제목 |
A MALDI-MS-based quantitative glycoprofiling method on a 96-well plate platform |
| 초록 |
Abnormal glycation changes are closely related to various human diseases, and the glycation profile has been used as a biomarker for the diagnosis of various diseases. Changes in protein glycosylation also have a direct effect on protein structure and function. In this study, we developed a high-throughput MALDI-MS based quantitative targeted glycomics (HT MALDI-QTaG) method for total N-glycan analysis. Despite the chemical derivatization process performed in 96-wells with built-in 10 kDa MWCO membrane filter, MALDI-MS showed excellent quantitative linearity (R2＞0.99) between the various amounts of target glycoprotein and MALDI peak intensity. We also confirmed relative quantitative reproducibility at different well locations in 96-well plates. In addition, the HT MALDI-QTaG method proposed in this study was successfully used for the analysis of bovine fetuin, human serum and Enbrel®. |
| 저자 |
황철환1, 박한규1, 송원석2, 안다희1, 김성민1, 김윤곤1
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| 소속 |
1숭실대, 2서울대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
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