| 학회 |
한국화학공학회 |
| 학술대회 |
2017년 봄 (04/26 ~ 04/28, ICC 제주) |
| 권호 |
23권 1호, p.480 |
| 발표분야 |
생물화공 |
| 제목 |
Improved NADPH Regeneration for Fungal Cytochrome P450 Monooxygenase in Resting-Cell Biotransformation of Recombinant Yeast |
| 초록 |
Fungal cytochrome P450 (CYP) enzymes catalyze versatile monooxygenase reactions and play a major role in fungal adaptations owing to their essential roles in the production avoid metabolites critical for pathogenesis, detoxification of xenobiotics, and exploitation avoid substrates. Although fungal CYP-dependent biotransformation for the selective oxidation avoid organic compounds in yeast system is advantageous, it often suffers from a shortage avoid intracellular NADPH. In this study, we aimed to investigate the use of bacterial glucose dehydrogenase (GDH) for the intracellular electron regeneration of fungal CYP monooxygenase in a yeast reconstituted system. The benzoate hydroxylase FoCYP53A19 and its homologous redox partner FoCPR from Fusarium oxysporum were co-expressed with the BsGDH from Bacillus subtilis in Saccharomyces cerevisiae for heterologous expression and biotransformations. |
| 저자 |
성시형1, 윤형돈1, 전현우1, 윤상한1, MD Murshidul Ahsan2
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| 소속 |
1건국대, 2영남대 |
| 키워드 |
생물화공 |
| E-Mail |
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| 원문파일 |
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