| 초록 |
A thermogelling polymer (mP) is prepared by pyridine-dicarboxylate (PDC) connected poly (ethylene glycol)- poly(propylene glycol)-poly(ethylene glycol) triblock copolymers. Tonsil derived mesenchymal stem cells (TMSCs) incorporated in the mP hydrogel system, aggregated to form live cell spheroids on day 7. In contrast, the spheroid formation is blocked in the Fe3+-incorporating mP thermogel. The 3D mP culture system exhibits significantly enhanced stemness, angiogenic, and anti-inflammatory biomarkers in the growth medium compared to 2D-cultured system. In addition, the stem cell spheroid exhibits significantly greater biomarker expression for osteogenic, chondrogenic, and adipogenic differentiations in each induction medium. This study suggests that a simple injection of stem cells suspended in the current aqueous mP solution can lead to the spontaneous formation of stem cell spheroids with excellent multipotency and retention in the in situ formed thermogel. |
